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ORIGINAL ARTICLE
Year : 2015  |  Volume : 10  |  Issue : 1  |  Page : 7-10

Comparative measuring between fresh and stored Drabkin's reagent preparations on hemoglobin estimation


1 Department of Hematology and Blood Transfusion, Karary University, College of Medical Laboratory Sciences, Omdurman, Sudan
2 Faculty of Medical Laboratory Sciences, University of Gezira, Wad Madani, Sudan
3 Faculty of Medicine and Health Sciences, Omdurman Islamic University, Omdurman, Sudan
4 Department of Biochemistry, Omdurman Islamic University, Omdurman, Sudan

Correspondence Address:
Gad Allah Modawe
Department of Biochemistry, Faculty of Medicine and Health Sciences, Omdurman Islamic University, Omdurman
Sudan
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DOI: 10.4103/1858-5000.157502

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This pilot case control hospital base study was conducted to determine the effect of using stored Drabkin's reagent on hemoglobin estimation compared with freshly prepared Drabkin's reagent. Freshly 50 ethylenediaminetetraacetic acid venous blood samples were collected from volunteers from Omdurman Military Hospital, Sudan, the Hb estimation performed using manual method (Hemiglobincyanide or cyanmethemoglobin method), Drabkin's used was prepared in a manner (fresh, 4 days, 8 days and 12 days stored Drabkin's). Then the collected data were analyzed by SPSS computer program. The result shows that the mean concentration of Hb (g/dL) when estimated by prepared Drabkin's reagent for several interval, the fresh reagent result was 13.0 g/dL, 4 days was reagent 11.9 g/dL, 8 days reagent was 11.4 g/dL and 12 days reagent was 11.3 g/dL. The study reveals that the mean of Hb when measured by 4 days stored Drabkin's reagent were statically insignificant and significant when measured by 8 days and 12 days stored Drabkin's reagent (P = 0.05).


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